Association of Chronic Myelogenous (Basophilic) Leukemia and the BCR/ABL Mutation in a Yucatan Barrow ( Sus scrofa domestica)
May 21, 2021Mallory0 Comments
Continual myelogenous leukemia (CML) is a clonal proliferative dysfunction of the myeloid, megakaryocyte, and erythroid lineages. The onset and subsequent development of CML is well-described in people. There’s comparably little data surrounding CML development in veterinary species, together with Yucatan miniature swine which might be frequent for preclinical pharmaceutical and system testing. In people, greater than 90% of CML circumstances are related to a chromosomal translocation that ends in the Philadelphia gene (BCR/ABL mutation). On this report, the presence of the Philadelphia gene in a Yucatan burrow was confirmed in white blood cells collected previous to onset of medical indicators with primers designed from the human BCR/ABL sequence.
A 24 month previous, 70 kg, Yucatan barrow acquired a prefabricated bovine cortical bone xenograft following a unilateral zygomatic ostectomy for a preclinical examine. Full blood rely and serum chemistries have been carried out previous to and 28, 53, 106, and 129 days after facial surgical procedure. Fifty three days after surgical procedure, a bonemarrow biopsy was carried out attributable to anorexia, extreme basophilia, and delicate anemia. A discovering of a reasonable enhance in basophilic precursors in bonemarrow cytology was adopted by lymphocyte immunophenotyping through movement cytometry and RT-PCR amplification
Philadelphia gene in white blood cell samples from the affected barrow and an unaffected barrow in the identical remedy group. Bonemarrow, lymph node, liver, spleen, lung, kidney, and adrenal gland lesions of largely myeloblasts have been recognized after the affected barrow died 146 days after surgical procedure. Circulation cytometry confirmed lymphopenia and urged basophilia, and RT-PCR established the presence of the BCR/ABL gene.
The knowledge on this report confirms the presence of the BCR/ABL mutation and paperwork development of continual myelogenous (basophilic) leukemia from a continual part to a terminal blast disaster in an grownup Yucatan barrow. The pure incidence and development of CML related to the BCR/ABL mutation in miniature swine establishes potential for future porcine fashions of human CML. The knowledge additionally establishes a genetic take a look at to substantiate porcine CML to forestall inadvertent attribution of medical indicators to remedy issues throughout preclinical testing.
Induction of the differentiation of porcine bonemarrow mesenchymal stem cells into untimely hepatocyte-like cells in an oblique coculture system with main hepatocytes
Liver transplantation is presently the one choice for sufferers with end-stage liver illness. Thus, different alternate therapeutic methods are wanted. Bonemarrow mesenchymal stem cells (BM-MSCs) are nonhematopoietic cells current within the bonemarrow stroma that function precursors cells for numerous different cells. On this examine, we evaluated the differentiation of porcine BM-MSCs into hepatocyte-like cells utilizing three varieties of tradition techniques: hepatic induction medium (HIM), HIM/main hepatocyte tradition supernatant (HCS; 1:1 ratio), and a hepatocyte coculture system (HCCS; main hepatocytes within the higher chamber, and BM-MSCs within the decrease chamber).
Main hepatocytes have been remoted from anesthetized wholesome 1-month-old pigs by enzymatic digestion. Hepatic-specific marker expression (albumin [ALB], transferrin [TF], α-fetoprotein [AFP]), glycogen storage, low-density lipoprotein, and indocyanine inexperienced uptake have been evaluated. Upregulation of hepatic-specific markers (ALB, TF, and AFP) was noticed by real-time polymerase chain response within the HCCS group. Periodic acid-Schiff staining revealed enhanced glycogen storage in hepatocyte-like cells from the HCCS group in contrast with that from the HIM/HCS group.
Moreover, hepatocyte like-cells within the HCCS group confirmed improved LDL and ICG uptake than these within the different teams. Total, our present examine revealed that oblique coculture of main hepatocytes and BM-MSCs enhanced the differentiation efficacy of BM-MSCs into hepatocyte-like cells by unknown helpful soluble components, together with paracrine components.
Description, Staging and Quantification of Pulmonary Artery Angiophagy in a Massive Animal Mannequin of Continual Thromboembolic Pulmonary Hypertension
Angiophagy has been described as a non-fibrinolytic mechanism of pulmonary artery (PA) patency restoration after distal (<50 µm in diameter) pulmonary embolism in mice. We hypothesized that angiophagy might obtain muscularized PA patency restoration after pulmonary embolism in piglets and people. Angiophagy was outlined by pathological evaluation because the shifting of an embolic specimen from the lumen to the interstitium based on three phases in a pig mannequin of continual thromboembolic pulmonary hypertension (CTEPH) 6 to 10 weeks after embolization with enbucrilate: the embolic specimen is (I) coated by endothelial cells, (II) coated by endothelial cells and clean muscle cells, and (III) situated within the adventitia.
In animals, we noticed the three phases of the pulmonary angiophagy of enbucrilate emboli in <300 µm PA. Phases II and III have been noticed in 300 to 1000 μm PA, and solely Stage I used to be noticed in larger-diameter PA (>1000 μm). In lung samples from sufferers with histories of pulmonary embolisms, we noticed PA angiophagy stigma for embolic specimens derived from blood clots and from bonemarrow emboli. This examine gives an authentic pathological description and staging of PA angiophagy in a big animal mannequin of CTEPH and in people after pulmonary embolism.
Growing the migratory capability of the implanted mesenchymal stem cells (MSCs) is a serious problem in growing profitable cell transplantation therapies. However, the regulatory components concerned within the migration of BMMSCs stay largely unknown. On this examine, we studied the position of the peripherin (PRPH) gene in regulating the flexibility of Wuzhishan mini pig (WZSP) BMMSCs emigrate in vitro. 4 totally different shRNA vectors directed towards PRPH have been designed and transfected into BMMSCs. The vector with the most effective interference impact was chosen for use within the following experiments.
Description: Small intestine cancer, metastatic lymph nodes and normal tissue high density tissue microarray, 69 cases/208 core, with stage and grade data
Small intestine disease spectrum (small intestine cancer progression) tissue array
Description: Small Intestine tumor tissue lysate was prepared by homogenization in lysis buffer (10 mM HEPES pH7.9, 1.5 mM MgCl2, 10 mM KCl, 1 mM ethylenediaminetetraacetic acid, 10% glycerol, 1% NP-40, and a cocktail of protease inhibitors). Tissue and cell debris was removed by centrifugation. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.
Description: Human small intestine tissue cytoplasmic protein lysate was prepared by isolating the cytoplasmic protein from whole tissue homogenates using a proprietary technique. The human small intestine tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The cytoplasmic protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, glycerol, and a cocktail of protease inhibitors. For quality control purposes, the isolated small intestine tissue cytoplasmic protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated small intestine tissue cytoplasmic protein is then Western analyzed by GAPDH antibody, and the expression level is consistent with each lot.
Description: Human small intestine tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human small intestine tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated small intestine tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated small intestine tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal.
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Human small intestine tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The human small intestine tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the small intestine tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The small intestine tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.
Description: Human small intestine tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human small intestine tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated small intestine tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated small intestine tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal.
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Small intestine carcinoma test tissue array, with normal small intestine tissues, including pathology grade, TNM and clinical stage, 6 cases/24 cores, replacing T421a
cDNA from Liver Cirrhosis: Small Intestine: Duodenum
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
cDNA from Liver Cirrhosis: Small Intestine: Jejunum
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Matched Pair Tissue Slides - Small Intestine Tissue
Description: The Matched Pair Paraffin Tissue (MPPT) slides are designed for identifying tumor-specific/metastasis genes or proteins. Slices from normal and malignant tissues are mounted on each MPPT slide which can then be treated as a single histological slide for H&E staining, immunohistochemistry, or in situ hybridization. This format allows a rapid analysis of protein expression and localization across normal and cancerous tissue.
Multiple Species Tissue Slides - Small Intestine Tissue
Description: The Multiple Species Tissue Array (MSTA) slides were designed to study protein expression patterns in different cells and tissues from multiple species. Tissue slices from three different species are mounted on each MSTA slide which can then be treated as a single histological slide for H&E staining, immunohistochemistry, or in situ hybridization. This format allows a rapid analysis of protein expression and localization across different species. MSTA slides can also be used to quickly determine the species reactivity of a given antibody.
Probable Small Intestine Urate Exporter (SLC17A4) Antibody
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
cDNA from Human Diabetic Tissue: Small Intestine: Duodenum
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
cDNA from Human Diabetic Tissue: Small Intestine: Ileum
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
cDNA from Human Diabetic Tissue: Small Intestine: Jejunum
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
cDNA from Monkey (Rhesus) Normal Tissue: Small Intestine
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
cDNA from Monkey (Cynomolgus) Normal Tissue: Small Intestine
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Genomic DNA from Mouse Normal Tissue: Small Intestine
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Genomic DNA from Rat Normal Tissue: Small Intestine
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Glucose Transporter Type 5, Small Intestine (GLUT5) Antibody
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.
Total Protein from Liver Cirrhosis: Small Intestine: Duodenum
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.
Total Protein from Liver Cirrhosis: Small Intestine: Ileum
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.
Total Protein from Liver Cirrhosis: Small Intestine: Jejunum
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.
Total Protein from Mouse Normal Tissue: Small Intestine
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.
Membrane Protein from Human Tumor Tissue: Small Intestine
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.
Monkey (Cyno.) Normal Small Intestine Whole tissue lysate
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.
Total RNA from Mouse Normal Tissue: Small Intestine
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.
Frozen Tissue Section - Human Adult Normal: Small Intestine
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.
Paraffin Tissue Section - Human Adult Normal: Small Intestine
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.
Small intestine disease (cancer progression) spectrum tissue array
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
cDNA from Human Adult Normal Tissue: Small Intestine: Ileum
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
cDNA from Human Adult Normal Tissue: Small Intestine: Jejunum
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
cDNA from Monkey (Rhesus) Normal Tissue: Small Intestine: Duodenum
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
cDNA from Monkey (Cynomolgus) Normal Tissue: Small Intestine: Duodenum
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
cDNA from Monkey (Rhesus) Normal Tissue: Small Intestine: Ileum
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
cDNA from Monkey (Cynomolgus) Normal Tissue: Small Intestine: Ileum
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
cDNA from Monkey (Rhesus) Normal Tissue: Small Intestine: Jejunum
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
cDNA from Monkey (Cynomolgus) Normal Tissue: Small Intestine: Jejunum
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Total Protein from Human Adult Normal Tissue: Small Intestine
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.
Total Protein from Human Tumor Tissue: Small Intestine: Duodenum
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.
Total Protein from Human Diabetic Tissue: Small Intestine: Duodenum
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.
Total Protein from Human Diabetic Tissue: Small Intestine: Ileum
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.
Total Protein from Human Diabetic Tissue: Small Intestine: Jejunum
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.
Membrane Protein from Human Adult Normal Tissue: Small Intestine
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.
Total RNA from Monkey (Rhesus) Normal Tissue: Small Intestine
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.
Total RNA from Monkey (Cynomolgus) Normal Tissue: Small Intestine
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.
Total RNA from Human Adult Normal Tissue: Small Intestine
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.
Frozen Tissue Section - Human Diabetic Tissue: Small Intestine: Duodenum
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.
Frozen Tissue Section - Human Diabetic Tissue: Small Intestine: Ileum
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.
Frozen Tissue Section - Human Diabetic Tissue: Small Intestine: Jejunum
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.
Paraffin Tissue Section - Human Small Intestine Tumor: Duodenum Adenocarcinoma
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.
Paraffin Tissue Section - Human Diabetic Tissue: Small Intestine: Duodenum
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.
Paraffin Tissue Section - Human Diabetic Tissue: Small Intestine: Ileum
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.
Paraffin Tissue Section - Human Diabetic Tissue: Small Intestine: Jejunum
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.
Genomic DNA from Lupus: Small Intestine, from a single donor
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Total Protein from Human Adult Normal Tissue: Small Intestine: Duodenum
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.
Total Protein from Human Adult Normal Tissue: Small Intestine: Ileum
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.
Total Protein from Human Adult Normal Tissue: Small Intestine: Jejunum
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.
Membrane Protein from Human Adult Normal Tissue: Small Intestine: Duodenum
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.
Membrane Protein from Human Adult Normal Tissue: Small Intestine: Ileum
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.
Membrane Protein from Human Adult Normal Tissue: Small Intestine: Jejunum
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.
Total RNA from Monkey (Rhesus) Normal Tissue: Small Intestine: Duodenum
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.
Total RNA from Monkey (Cynomolgus) Normal Tissue: Small Intestine: Duodenum
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.
Total RNA from Monkey (Rhesus) Normal Tissue: Small Intestine: Ileum
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.
Total RNA from Human Adult Normal Tissue: Small Intestine: Duodenum
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.
Total RNA from Human Adult Normal Tissue: Small Intestine: Ileum
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.
Total RNA from Human Adult Normal Tissue: Small Intestine: Jejunum
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.
Human, Mouse, Rat - Small Intestine Tissue Array (5 slides/pk)
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Human Small Intestine Tissue Slide (Squamous Cell Carcinoma) (5 slides/pk)
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Genomic DNA - Human Diabetic Tissue: Small Intestine: Ileum, from a single donor
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Genomic DNA - Human Diabetic Tissue: Small Intestine: Jejunum, from a single donor
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Total RNA from Human Adult Normal Tissue 5 Donor Pool: Small Intestine
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.
Cynomolgus Monkey: Small intestine (duodenum) (single section per slide) (5 slides/pack)
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Matched Pair (Normal and Carcinoma) Human Small Intestine Tissue Array (5 slides/pk)
Description: Small intestine(most of duodenum) adenocarcinoma tissue array with matched NAT or AT as control, including TNM, clinical stage and pathology grade, 50 cases/ 150 cores
The expression degree of PRPH in BMMSCs was decided by quantitative real-time PCR and western blot evaluation. The migration capability of the BMMSCs was estimated utilizing a scratch assay, a transwell in vitro migration mannequin assay, and filamentous actin staining. The outcomes confirmed that shRNA-mediated knockdown of the expression of the PRPH gene in BMMSCs lowered the flexibility of those cells emigrate. Total, these outcomes illustrate that the PRPH gene regulates the migration of BMMSCs within the WZSP.
