Laparoscopy for the Treatment of Congenital Hernia: Use of Surgical Meshes and Mesenchymal Stem Cells in a Clinically Relevant Animal Model

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Laparoscopy for the Treatment of Congenital Hernia: Use of Surgical Meshes and Mesenchymal Stem Cells in a Clinically Relevant Animal Model

Greater than a century has handed for the reason that first surgical mesh for hernia restore was developed, and, thus far, that is nonetheless probably the most broadly used technique regardless of the good variety of problems it poses. The aim of this examine was to mix stem cell remedy and laparoscopy for the remedy of congenital hernia in a swine animal mannequin. Porcine bone marrow-derived mesenchymal stem cells (MSCs) have been seeded on polypropylene surgical meshes utilizing a fibrin sealant resolution as a car. Meshes with (cell group) or with out (management group) MSCs have been implanted via laparoscopy in Giant White pigs with congenital stomach hernia after the approximation of hernia borders (implantation day).

A successive laparoscopic biopsy of the mesh and its surrounding tissues was carried out every week after implantation, and surgical meshes have been excised a month after implantation. Ultrasonography was used to measure hernia sizes. Move cytometry, histological, and gene expression analyses of the biopsy and necropsy samples have been carried out. The fibrin sealant resolution was straightforward to arrange and preserved the viability of MSCs within the surgical meshes. Ultrasonography demonstrated a big discount in hernia measurement 1 week after implantation within the cell group relative to that on the day of implantation (p < 0.05).

Move cytometry of the mesh-infiltrated cells confirmed a non-significant enhance of M2 macrophages when the cell group was in contrast with the management group 1 week after implantation. A major lower within the gene expression of VEGF and a big enhance in TNF expression have been decided within the cell group 1 month after implantation in contrast with gene expressions within the management group (p < 0.05).

Right here, we suggest a simple and possible technique to mix stem cell remedy and minimally invasive surgical strategies for hernia restore. On this examine, stem cell remedy didn’t present an awesome immunomodulatory or regenerative impact in overcoming hernia-related problems. Nevertheless, our clinically related animal mannequin with congenital hernia carefully resembles the medical human situation. Additional research needs to be targeted on this priceless animal mannequin to judge stem cell therapies in hernia surgical procedure.

Improvement and Validation of a Methodology for the Detection and Quantification of Antidepressants and Antipsychotics in Bone Samples by GC-MS

Bone marrow is the tissue contained contained in the bones and might be thought-about one of many potential various tissues in forensic toxicology. This matrix may very well be notably helpful in these instances the place the routine pattern shouldn’t be out there attributable to a sophisticated state of decomposition or skeletonization of the corpse. Intention of this examine was develop, validate and apply an analytical technique of extraction and evaluation of various antidepressants and antipsychotics, generally utilized in remedy, from spiked pig ribs.

Pig ribs, every of 5 g and 5 cm lengthy, have been spiked at three focus ranges (100, 200 and 500 ng/g). For every focus, ten pig ribs have been ready. The strategy entails the leaching by ethanol at totally different pHs of 9 medicine from the within of ribs, specifically from the bone marrow, with out the fragmentation of bone tissue. Following a liquid-liquid purification and extraction, evaluation was carried out by fuel chromatography coupled with mass spectrometry in SIM mode.

For technique validation was assessed linearity, sensitivity, precision and accuracy, matrix interferences and, lastly, carryover. Analytical technique efficiency was acceptable respect to acceptance standards for validation. No matrix interferences have been detected; because of this, it’s doable to affirm that this technique has a great selectivity. Furthermore, the strategy was not affected by carryover. Contemplating that the examine performed on pig ribs has given encouraging outcomes, it may be assumed that this technique can be utilized in forensic toxicological protocols (human post-mortem instances) as various to basic procedures.

Laparoscopy for the Treatment of Congenital Hernia: Use of Surgical Meshes and Mesenchymal Stem Cells in a Clinically Relevant Animal Model

Electrospun Fibers Immobilized with BMP-2 Mediated by Polydopamine Mixed with Autogenous Tendon to Restore Developmental Dysplasia of the Hip in a Porcine Mannequin

 Developmental dysplasia of the hip (DDH) can enhance the strain between the joints, which causes secondary hip osteoarthritis. The intention of the current examine was to manufacture poly(D, L-lactic acid)-poly(ethylene glycol)-poly(D, L-lactic acid) (PELA) electrospun fibrous scaffolds, immobilized with bone morphogenetic protein-2 (BMP-2), to restore the acetabulum defects. The traits of PELA electrospun have been analyzed utilizing scanning electron microscopy, the discharge kinetics of BMP-2 in vitro have been analyzed utilizing enzyme-linked immunosorbent assays.
Human mesenchymal stem cells (hMSCs) have been used for in vitro experiments, the biocompatibility of the electrospinning supplies was investigated utilizing a cell counting kit-8 (CCK-8) equipment, and osteogenic differentiation was examined by way of alkaline phosphatase (ALP) exercise and relative gene expression. Eighteen miniature pig animal fashions have been used within the in vivo experiment. The pigs have been sacrificed at 24 weeks after surgical procedure, and the reconstructed acetabulum was evaluated utilizing histological sections.
Structural evaluation revealed that the diameter of the PELA electrospun fiber was 0.8195 ± 0.16 μm. The PELA electrospun fiber supplies confirmed good hydrophilicity and biocompatibility and will constantly launch BMP-2 inside 27 days: 16.07 ± 0.11 ng of BMP-2 was launched from the scaffold. A complete of sixteen implants totally stuffed the defects, and hematoxylin and eosin staining and Goldner’s trichrome staining confirmed that the straightforward tendon group (T group) was principally fibrous tissues, numerous fibroblasts and small quantities of chondrocytes have been noticed within the polydopamine-coated electrospun fiber group (DP group).

F4/80. PAN MACROPHAGES, Antibody

GWB-8969B1 0.1 mg Ask for price

Macrophages (pan) mouse monoclonal antibody, clone RM1, Purified

BM4004 100 µg Ask for price

Monocytes/Macrophages Mouse, Antibody

GWB-F0FC0B 0.2 mg Ask for price

Mouse Macrophages/Monocytes Antibody

GWB-A95ECA 0.25 mg Ask for price

Mouse Macrophages/Monocytes Antibody

GWB-97F4EF 0.025 mg Ask for price

C57BL/6 Mouse Peritoneal Macrophages

ABC-TC3225 1 vial Ask for price
Description: Mouse Peritoneal Macrophages are derived from the thioglycollate-elicited peritoneal cavity of pathogen-free laboratory adult mice. Mouse Peritoneal Macrophages are negative for bacteria, yeast, fungi, and mycoplasma. Each vial contains at least 8 x10^6 cells and is delivered frozen. Cells can be expanded on a multiwell culture plate ready for experiments under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended. Mouse Peritoneal Macrophages are tested for expression of markers using antibodies, CD11b by flow cytometry. Standard biochemical procedures performed with cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, flow cytometry or generating cell derivatives for desired research applications.

CD-1 Mouse Bone Marrow Macrophages

ABC-TC3276 1 vial Ask for price
Description: Mouse bone marrow macrophages (MACBM) are derived from the tibias and femurs of pathogen-free laboratory adult mice. Mouse bone marrow macrophages are grown in 100 mm treated tissue culture dish and incubated in Culture Complete Growth Medium for 10-15 doubling times.

BALB/c Mouse Bone Marrow Macrophages

ABC-TC3058 1 vial Ask for price
Description: Mouse bone marrow macrophages (MPBMMACs) are derived from the tibias and femurs of pathogen-free laboratory adult mice. MPBMMACs are grown in 100 mm treated tissue culture dish and incubated in Culture Complete Growth Medium for 10-15 days.

Mouse Macrophages / Monocytes Antibody (RPE)

GWB-Q01554 100 TESTS Ask for price

Mouse Macrophages / Monocytes Antibody (RPE)

GWB-Q01555 25 TESTS Ask for price

C57BL/6 Mouse Bone Marrow Macrophages

ABC-TC3171 1 vial Ask for price
Description: Mouse Bone Marrow Macrophages are derived from the tibias and femurs of pathogen-free laboratory adult mice. Mouse Bone Marrow Macrophages are grown in 100 mm treated tissue culture dish and incubated in Gentaur's Culture Medium for 7-10 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 6x10^6 cells and is delivered frozen.Mouse Bone Marrow Macrophages are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded on a multiwell culture plate ready for experiments under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Mouse Bone Marrow Macrophages are tested for expression of markers using antibodies, CD11b by flow cytometry. Standard biochemical procedures performed with cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, flow cytometry or generating cell derivatives for desired research applications.

Mouse Macrophages / Monocytes Antibody (FITC)

GWB-Q01551 0.1 mg Ask for price

Mouse Macrophages / Monocytes Antibody (FITC)

GWB-Q01552 50 ug Ask for price

Mouse Macrophages / Monocytes Antibody (FITC)

GWB-Q01553 25 ug Ask for price

C57BL/10J Mouse Bone Marrow Macrophages

ABC-TC3143 1 vial Ask for price
Description: Mouse bone marrow macrophages (MPBMMACs) are derived from the tibias and femurs of pathogen-free laboratory adult mice. MPBMMACs are grown in 100 mm treated tissue culture dish and incubated in Culture Complete Growth Medium for 10-15 days.

C57BL/6 Mouse Peritoneal Macrophages (TE)

ABC-TC3226 1 vial Ask for price
Description: Mouse Peritoneal Macrophages are derived from the thioglycollate-elicited peritoneal cavity of pathogen-free laboratory adult mice. Mouse Peritoneal Macrophages are negative for bacteria, yeast, fungi, and mycoplasma. Each vial contains at least 8 x10^6 cells and is delivered frozen.Cells can be expanded on a multiwell culture platetandard biochemical procedures performed with cell ready for experiments under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Mouse Peritoneal Macrophages are tested for expression of markers using antibodies, CD11b by flow cytometry. Standard biochemical procedures performed with cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, flow cytometry or generating cell derivatives for desired research applications.

B6129SF2/J Mouse Bone Marrow Macrophages

ABC-TC3038 1 vial Ask for price
Description: Mouse bone marrow macrophages (MPBMMACs) are derived from the tibias and femurs of pathogen-free laboratory adult mice. MPBMMACs are grown in 100 mm treated tissue culture dish and incubated in Culture Complete Growth Medium for 10-15 days.

Macrophages mouse monoclonal antibody, clone 25F9, FITC

BM4022FS 100 µg Ask for price

Macrophages Antibody

GWB-36F900 0.2 ml Ask for price

Macrophages Antibody

GWB-45D900 0.02 mg Ask for price

Macrophages Antibody

GWB-B68A2C 2 ml Ask for price

Macrophages Antibody

GWB-3243CF 0.2 ml Ask for price

Macrophages Antibody

GWB-33EA8D 0.2 mg Ask for price

Macrophages Antibody

GWB-DAD90D 0.1 mg Ask for price

MACROPHAGES, Antibody

GWB-DE8FCB 0.1 mg Ask for price

Macrophages mouse monoclonal antibody, clone BA4D5, FITC

AM05890FC-N 100 µg Ask for price

Macrophages mouse monoclonal antibody, clone PM1, Purified

AM26132PU-N 200 µg Ask for price

BALB/c Mouse Resident Peritoneal Macrophages

ABC-TC3100 1 vial Ask for price
Description: Mouse Resident Peritoneal Macrophages are derived from the peritoneal cavity of pathogen-free laboratory adult mice. Each vial contains at least 8 x10^6 cells and is delivered frozen.Mouse Resident Peritoneal Macrophages are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded on a multiwell culture plate ready for experiments under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Mouse Resident Peritoneal Macrophages are tested for expression of markers using antibodies, CD11b by flow cytometry. Standard biochemical procedures performed with cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, flow cytometry or generating cell derivatives for desired research applications.

C57BL/6 Mouse Resident Peritoneal Macrophages

ABC-TC3240 1 vial Ask for price
Description: Mouse Resident Peritoneal Macrophages are derived from the peritoneal cavity of pathogen-free laboratory adult mice. Each vial contains at least 8 x10^6 cells and is delivered frozen.Mouse Resident Peritoneal Macrophages are negative for bacteria, yeast, fungi, and mycoplasma.Cells can be expanded on a multiwell culture platetandard biochemical procedures performed with cell ready for experiments under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Mouse Resident Peritoneal Macrophages are tested for expression of markers using antibodies, CD11b by flow cytometry. Standard biochemical procedures performed with cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, flow cytometry or generating cell derivatives for desired research applications.

Macrophages mouse monoclonal antibody, clone MAC 387, Purified

AM21060PU-N 50 µg Ask for price

Anti-Mouse Macrophages Antibody

103-M101 100 µg
EUR 399
Description: This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a rat immunized with isolated mouse macrophages).

Mouse Anti Human Macrophages Monoclonal Antibody

DMABT-49597MH 0.2 ml
EUR 483.6

Mouse Anti Human Macrophages Monoclonal Antibody

DMABT-49598MH 2 ml
EUR 1008

Macrophages mouse monoclonal antibody, clone EP-3, Purified

AM32125PU-N 100 µg Ask for price

Macrophages mouse monoclonal antibody, clone LN-5, Purified

AM32128PU-N 100 µg Ask for price

Immortalized Mouse Bone Marrow Macrophages-SV40

CSC-I2161Z One Frozen vial Ask for price

Macrophages antibody (PE)

61R-1268 100 ug
EUR 418
Description: Mouse monoclonal Macrophages antibody (PE)

Macrophages mouse monoclonal antibody, clone PM-2K, Purified

BM4037 100 µg Ask for price

Macrophages mouse monoclonal antibody, clone PM-2K, Purified

BM4037B 100 µg Ask for price

Rat Alveolar Macrophages

ABC-TC5506 1 vial Ask for price
Description: Rat Priamry Alveolar Macrophages are derived from the lung of 6-8 weeks old Sprague–Dawley Rats. Each vial contains at least 0.5x106 cells. Rat Alveolar Macrophages are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded on a multiwell culture plate ready for experiments under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.

Macrophages Antibody (RPE)

GWB-Q01144 100 TESTS Ask for price

Macrophages Antibody (FITC)

GWB-4562DA 0.1 mg Ask for price

Macrophages Antibody (FITC)

GWB-665EFD 0.02 mg Ask for price

Macrophages mouse monoclonal antibody, clone AnP95, Aff - Purified

AM26064PU-N 100 µg Ask for price

Mouse Anti Pig Macrophages Monoclonal Antibody,FITC

DMABT-52020MP 0.1 mg
EUR 1057.2

MATURE MACROPHAGES, Antibody

GWB-ED7BB8 0.1 mg Ask for price

MATURE MACROPHAGES, Antibody

GWB-8E4353 0.05 mg Ask for price

Mouse Anti Guinea Pig Macrophages Monoclonal Antibody

DMABT-51930MG 2 ml
EUR 920.4

Rat Bone Marrow Macrophages

ABC-TC4086 1 vial Ask for price
Description: Rat bone marrow macrophages (RPBMMACs) are derived from the tibias and femurs of Sprague–Dawley rats. RPBMMACs are grown in 100 mm treated tissue culture dish and incubated in Culture Complete Growth Medium for 10-15 days. Cultures are then expanded.

Rat Monoclonal anti-mouse Macrophages

mAP-0029 100ug
EUR 250

Rat Anti Mouse Macrophages/Monocytes Monoclonal Antibody

DMABT-54496RM 25 µg
EUR 446.4

Rat Anti Mouse Macrophages/Monocytes Monoclonal Antibody

DMABT-54497RM 0.25 mg
EUR 920.4

Pig Macrophages Antibody (FITC)

GWB-Q01229 0.1 mg Ask for price

Rat macrophages (CD163), Antibody

GWB-5ACF20 0.25 mg Ask for price

Guinea Pig Macrophages Antibody

GWB-Q00951 2 ml Ask for price

Human iPSC-derived macrophages

CSC-00834L One Frozen vial Ask for price

Immortalized Pig Macrophages - SV40

T9260 1x10^6 cells / 1.0 ml Ask for price

Rat Anti Mouse Macrophages/Monocytes Monoclonal Antibody,RPE

DMABT-54498RM 25 TEST
EUR 514.8

Rat Anti Mouse Macrophages/Monocytes Monoclonal Antibody,RPE

DMABT-54499RM 100 TEST
EUR 795.6

Macrophages / Monocytes mouse monoclonal antibody, clone MR-1

AM05899SU-N 2 ml Ask for price

MACROPHAGES Antibody (Low Endotoxin)

GWB-705CC2 0.5 mg Ask for price

Rat Anti Mouse Macrophages/Monocytes Monoclonal Antibody,FITC

DMABT-54493RM 25 µg
EUR 534

Rat Anti Mouse Macrophages/Monocytes Monoclonal Antibody,FITC

DMABT-54494RM 50 µg
EUR 795.6

Rat Anti Mouse Macrophages/Monocytes Monoclonal Antibody,FITC

DMABT-54495RM 0.1 mg
EUR 889.2

Rat Resident Peritoneal Macrophages

ABC-TC4211 1 vial Ask for price
Description: Rat Resident Peritoneal Macrophages are derived from the peritoneal cavity of Sprague-Dawley Rats. Prior to shipping, cells are immediately cryo-preserved in vials. Each vial contains at least 9 x10^6 cells and is delivered frozen.Rat Resident Peritoneal Macrophages were tested for expression of markers using antibodies, CD11b by flow cytometry. Rat Resident Peritoneal Macrophages are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded on a multiwell culture plate ready for experiments under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, flow cytometry or generating cell derivatives for desired research applications.

C57BL/6 Mouse Peritoneal Macrophages-Thioglycollate-elicited

ABC-TC3227 1 vial Ask for price
Description: Mouse peritoneal macrophages (MPMAC) are derived from the thioglycollate-elicited peritoneal cavity of pathogen-free laboratory adult mice. MPMAC are negative for bacteria, yeast, fungi, and mycoplasma.

BALB/c Mouse Peritoneal Macrophages-Thioglycollate-elicited

ABC-TC3096 1 vial Ask for price
Description: Mouse Peritoneal Macrophages are derived from the thioglycollate-elicited peritoneal cavity of pathogen-free laboratory adult mice. Mouse Peritoneal Macrophages are negative for bacteria, yeast, fungi, and mycoplasma. Each vial contains at least 8 x10^6 cells and is delivered frozen.Cells can be expanded on a multiwell culture plate ready for experiments under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Mouse Peritoneal Macrophages are tested for expression of markers using antibodies, CD11b by flow cytometry. Standard biochemical procedures performed with cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, flow cytometry or generating cell derivatives for desired research applications.

Macrophages And Neutrophils Antibody

GWB-C29733 200 TESTS Ask for price

MONOCYTES MACROPHAGES. SUBSET, Antibody

GWB-CA34C3 0.1 mg Ask for price

Mouse Anti Human Macrophage (Tissue And Proliferative Disorders) Monoclonal Antibody

DMABT-52185MH 0.5 ml
EUR 1226.4

Chicken Monocytes/Macrophages Antibody

GWB-Q00905 0.25 mg Ask for price

OASA11733-100UG - PIG MACROPHAGES Antibody

OASA11733-100UG 0.1mg
EUR 319

Chicken Macrophages (+ Monocytes) mouse monoclonal antibody, clone KUL01, PE

AM08143RP-N 100 µg Ask for price

FITC CONJUGATED MATURE MACROPHAGES, Antibody

GWB-7E3823 0.1 mg Ask for price

Macrophages rabbit polyclonal antibody, Serum

AP31652SU-N 5 ml Ask for price

Macrophages rabbit polyclonal antibody, Serum

AP31653SU-N 5 ml Ask for price

Macrophages rabbit polyclonal antibody, Serum

AP31654SU-N 2 ml Ask for price

Chicken Macrophages (+ Monocytes) mouse monoclonal antibody, clone KUL01, FITC

AM08143FC-N 500 µg Ask for price

F4/80 ( monocytes / Macrophages) murine, Antibody

GWB-536F90 0.25 mg Ask for price

Monkey Bone Marrow Macrophages (Frozen Cells)

ABC-TC5534 1 vial Ask for price
Description: Monkey Bone Marrow Macrophages are derived from the tibias or femurs of Cynomolgus Monkey. Cells at passage 0 are cryo-preserved in vials. Each vial contains 6x106 cells and is delivered frozen. Cells are negative for bacteria, yeast, fungi, and mycoplasma and tested for expression of markers using antibody, CD11b by flow cytometry. Cells can be expanded on a multiwell culture plate ready for experiments under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.

OASA05698-25UG - MACROPHAGES/MONOCYTES Antibody

OASA05698-25UG 25ug
EUR 149

CD14,(Monocytes/Macrophages) Antibody, Concentrate

MAB473C 0.2ml
EUR 435

Chicken Macrophages (+ Monocytes) mouse monoclonal antibody, clone KUL01, Purified

AM08143PU-N 500 µg Ask for price

OASA11517-250UG - MONOCYTES/MACROPHAGES Antibody

OASA11517-250UG 0.25mg
EUR 479

anti-CD26 T/B lymphocytes and macrophages

512-A-01mg 0,1 mg
EUR 321
Description: anti-CD26 Activated T, B lymphocytes and macrophages

anti-CD26 T/B lymphocytes and macrophages

512-A-1000ug 1000 ug
EUR 1539
Description: anti-CD26 Activated T, B lymphocytes and macrophages

OASA00755-2ML - GUINEA PIG MACROPHAGES Antibody

OASA00755-2ML 2ml
EUR 539

Macrophages (Haematopoiesis associated) mouse monoclonal antibody, clone 25F9, FITC

BM4022F 200 µg Ask for price

Interdigitating cells (access. T-cell Macrophages) mouse monoclonal antibody, clone X-12, Purified

BM4056 100 µg Ask for price

Macrophages (Haematopoiesis associated) mouse monoclonal antibody, clone 25F9, Biotin

BM4022B 200 µg Ask for price

Macrophages (Haematopoiesis associated) mouse monoclonal antibody, clone 25F9, Biotin

BM4022BS 50 µg Ask for price

Macrophages (ED2-like antigen) mouse monoclonal antibody, clone HIS36, Supernatant

AM39058SU-N 1 ml Ask for price

macrophages and Interdigitating Cells Rat, Antibody

GWB-1E0670 0.1 mg Ask for price

Macrophages rabbit polyclonal antibody, Aff - Purified

CL194P 1 ml Ask for price

Human Peripheral Blood Monocyte Derived Macrophages

ABC-TC4009 1 vial Ask for price
Description: NPB-Macrophages are derived from negatively-selected peripheral blood monocytes and stimulated with M-CSF and IL-4 growth factors and cultured for 6-7 day.

Macrophages (Haematopoiesis associated) mouse monoclonal antibody, clone 25F9, Purified

BM4022 100 µg Ask for price

Rat Peritoneal Macrophages-Thioglycollate-elicited

ABC-TC4193 1 vial Ask for price
Description: Rat Peritoneal Macrophages are derived from the thioglycollate-elicited peritoneal cavity of Sprague-Dawley Rats. Prior to shipping, cells are immediately cryo-preserved in vials. Each vial contains at least 9 x10^6 cells and is delivered frozen.Rat Peritoneal Macrophages were tested for expression of markers using antibodies, CD11b by flow cytometry. Rat Peritoneal Macrophages are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded on a multiwell culture plate ready for experiments under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, flow cytometry or generating cell derivatives for desired research applications.

Anti-F4/80 ( monocytes / Macrophages), murine Antibody

Y103412 250 µg
EUR 680

Macrophages (Marginal Metallophils) mouse monoclonal antibody, clone TRPM-3, Purified

BM4118 100 µg Ask for price

CD11B Rat macrophages / microglial cells (C3bi), Antibody

GWB-5A19A2 0.5 mg Ask for price

Rat Peritoneal Macrophages (fresh cells in suspension)

ABC-TC4192 1 vial Ask for price
Description: Rat peritoneal macrophages (RPMAC) are derived from the peritoneal cavity of Sprague–Dawley rats. RPMAC are negative for bacteria, yeast, fungi, and mycoplasma.

CD14,(Monocytes/Macrophages) Antibody, Ready-To-Use

MAB473P 5ml
EUR 435

OASA03719-100UG - Macrophages Antibody - FITC Conjugated

OASA03719-100UG 0.1mg
EUR 589

INTERMEDIATE STAGE INFLAMMATORY MACROPHAGES (CD163), Antibody

GWB-CED810 0.1 mg Ask for price

anti-CD26 Activated T, B lymphocytes and macrophages

512-A 1 MG
EUR 850

anti-CD26 Activated T, B lymphocytes and macrophages

512-A-100ugvial 100 ug/vial
EUR 150
Description: anti-CD26 Activated T, B lymphocytes and macrophages

OASA05697-250UG - Rat ANTI MACROPHAGES/MONOCYTES Antibody

OASA05697-250UG 0.25mg
EUR 499

AgileGrinder? Tissue Grinder (fixed speed 12000 rpm)

ACT-AG12000 each
EUR 826.8

AgileGrinder? Tissue Grinder (fixed speed 1500 rpm)

ACT-AG1500 each
EUR 710.4

OASA05702-100T - Macrophages/Monocytes Antibody - PE Conjugated

OASA05702-100T 100Tests
EUR 519

Macrophage Antibody (pan)

V3066-100UG 100 ug
EUR 424.15
Description: This mAb stains the cytoplasm of macrophages and histiocytes in hematopoietic organs, Kupffer s cells of the liver and Langerhan s cells of the skin. Macrophages comprise of many forms of mononuclear phagocytes found in tissues. Mononuclear phagocytes arise from hematopoietic stem cells in the bone marrow. After passing through the monoblast and pro-monocyte states of the monocyte stage, they enter the blood, where they circulate for about 40 hours. They then enter tissues and increase in size, phagocytic activity, and lysosomal enzyme content becoming macrophages. Among the functions of macrophages are nonspecific phagocytosis and pinocytosis, specific phagocytosis of opsonized microorganisms mediated by Fc receptors and complement receptors, killing of ingested microorganisms, digestion and presentation of antigens to T and B lymphocytes, and secretion of a large number of diverse products, including many enzymes including lysozyme and collagenases, several complement components and coagulation factors, some prostaglandins and leukotrienes, and many regulatory molecules (Interferon, Interleukin 1). LN-5 selectively stains human sebaceous glands in formalin-fixed, paraffin-embedded skin samples. Undifferentiated sebocyte progenitors are negative, and only sebocytes from the onset of their differentiation reveal positive cytoplasmic staining. Since there are very few selective and easy-to-use markers of sebaceous glands, LN-5 antibody can offer a simple and relatively specific way to detect human sebocytes from the onset of their.

Macrophage Antibody (pan)

V3066-20UG 20 ug
EUR 186.15
Description: This mAb stains the cytoplasm of macrophages and histiocytes in hematopoietic organs, Kupffer s cells of the liver and Langerhan s cells of the skin. Macrophages comprise of many forms of mononuclear phagocytes found in tissues. Mononuclear phagocytes arise from hematopoietic stem cells in the bone marrow. After passing through the monoblast and pro-monocyte states of the monocyte stage, they enter the blood, where they circulate for about 40 hours. They then enter tissues and increase in size, phagocytic activity, and lysosomal enzyme content becoming macrophages. Among the functions of macrophages are nonspecific phagocytosis and pinocytosis, specific phagocytosis of opsonized microorganisms mediated by Fc receptors and complement receptors, killing of ingested microorganisms, digestion and presentation of antigens to T and B lymphocytes, and secretion of a large number of diverse products, including many enzymes including lysozyme and collagenases, several complement components and coagulation factors, some prostaglandins and leukotrienes, and many regulatory molecules (Interferon, Interleukin 1). LN-5 selectively stains human sebaceous glands in formalin-fixed, paraffin-embedded skin samples. Undifferentiated sebocyte progenitors are negative, and only sebocytes from the onset of their differentiation reveal positive cytoplasmic staining. Since there are very few selective and easy-to-use markers of sebaceous glands, LN-5 antibody can offer a simple and relatively specific way to detect human sebocytes from the onset of their.

Macrophage Antibody (pan)

V3066SAF-100UG 100 ug
EUR 424.15
Description: This mAb stains the cytoplasm of macrophages and histiocytes in hematopoietic organs, Kupffer s cells of the liver and Langerhan s cells of the skin. Macrophages comprise of many forms of mononuclear phagocytes found in tissues. Mononuclear phagocytes arise from hematopoietic stem cells in the bone marrow. After passing through the monoblast and pro-monocyte states of the monocyte stage, they enter the blood, where they circulate for about 40 hours. They then enter tissues and increase in size, phagocytic activity, and lysosomal enzyme content becoming macrophages. Among the functions of macrophages are nonspecific phagocytosis and pinocytosis, specific phagocytosis of opsonized microorganisms mediated by Fc receptors and complement receptors, killing of ingested microorganisms, digestion and presentation of antigens to T and B lymphocytes, and secretion of a large number of diverse products, including many enzymes including lysozyme and collagenases, several complement components and coagulation factors, some prostaglandins and leukotrienes, and many regulatory molecules (Interferon, Interleukin 1). LN-5 selectively stains human sebaceous glands in formalin-fixed, paraffin-embedded skin samples. Undifferentiated sebocyte progenitors are negative, and only sebocytes from the onset of their differentiation reveal positive cytoplasmic staining. Since there are very few selective and easy-to-use markers of sebaceous glands, LN-5 antibody can offer a simple and relatively specific way to detect human sebocytes from the onset of their.

Rat Anti Human Macrophages And Neutrophils Monoclonal Antibody

DMABT-49599RH 200 TEST
EUR 920.4

OASA05699-100UG - Macrophages/Monocytes Antibody - FITC Conjugated

OASA05699-100UG 0.1mg
EUR 459

Macrophages / Monocytes rat monoclonal antibody, clone MOMA-2, PE

SM065R 100 Tests Ask for price

Macrophages / Monocytes rat monoclonal antibody, clone MOMA-2, FITC

SM065F 100 µg Ask for price

Macrophages / Monocytes rat monoclonal antibody, clone MOMA-2, FITC

SM065FS 50 µg Ask for price

Macrophages / Monocytes rat monoclonal antibody, clone MOMA-2, Biotin

SM065B 100 µg Ask for price

Activated Macrophages rat monoclonal antibody, clone 88a, Aff - Purified

AM26130PU-N 100 µg Ask for price

Macrophages / Monocytes rat monoclonal antibody, clone MOMA-2, Purified

SM065P 200 µg Ask for price

Macrophages (Haematopoiesis associated) rat monoclonal antibody, clone ER-HR3, Purified

BM4016 500 µg Ask for price

Macrophages (Haematopoiesis associated) rat monoclonal antibody, clone ER-HR3, Purified

BM4016T 100 µg Ask for price

Cell Meterâ„¢ Fixed Cell and Tissue TUNEL Apoptosis Assay Kit *Red Fluorescence*

22853-25Tests 25 Tests
EUR 425
Description: Cell Meterâ„¢ TUNEL apoptosis assay kit provides a robust tool for conveniently detecting apoptosis caused by DNA fragmentation.

Cell Meterâ„¢ Fixed Cell and Tissue TUNEL Apoptosis Assay Kit *Green Fluorescence*

22851-25Tests 25 Tests
EUR 425
Description: Cell Meterâ„¢ Fixed Cell and Tissue TUNEL Apoptosis Assay Kit provides a robust tool for conveniently detecting DNA fragmentation caused by apoptosis.

Cell Meterâ„¢ Fixed Cell and Tissue TUNEL Apoptosis Assay Kit *Blue Fluorescence*

22857-25Tests 25 Tests
EUR 425
Description: Cell Meterâ„¢ TUNEL apoptosis assay kit provides a robust tool for conveniently detecting apoptosis caused by DNA fragmentation.

Cell Meterâ„¢ Fixed Cell and Tissue TUNEL Apoptosis Assay Kit *Deep Red Fluorescence*

22855-25Tests 25 Tests
EUR 425
Description: Cell Meterâ„¢ TUNEL apoptosis assay kit provides a robust tool for conveniently detecting apoptosis caused by DNA fragmentation.

Mst1r ELISA Kit| Mouse Macrophage-stimulating protein receptor

EF015429 96 Tests
EUR 826.8

Mouse Macrophage-stimulating protein receptor (MST1R) ELISA Kit

abx389794-96tests 96 tests
EUR 1093.2

Protein Extraction for Fixed and Embedded Tissues

P525-20 - Ask for price

CD-1 Mouse Macrophage

ABC-TC3296 1 vial Ask for price
Description: Macrophages are cells produced by the differentiation of monocytes in tissues. Macrophages are large cells found within many types of tissue. They derive from monocytes that have entered tissues.

Mouse Macrophage antibody

20R-MR010 1 ml
EUR 434
Description: Rabbit polyclonal Mouse Macrophage antibody

Mouse Macrophage antibody

20R-MR014 5 ml
EUR 660
Description: Rabbit polyclonal Mouse Macrophage antibody

Mouse Macrophage antibody

20R-MR015 10 mg
EUR 399
Description: Rabbit polyclonal Mouse Macrophage antibody

Mouse Macrophage antibody

20R-MR029 5 mg
EUR 471
Description: Rabbit polyclonal Mouse Macrophage antibody

Mouse Macrophage antibody (FITC)

60R-MR006FT 2 mg
EUR 532
Description: Rabbit polyclonal Mouse Macrophage antibody (FITC) conjugated

Anti- Macrophage Mouse Antibody

GWB-3B92D9 5 ml Ask for price

Mouse iNOS (macrophage) cDNA probe

iNOS61-D-2 2 ug
EUR 534

Primary Mouse Thymic Macrophage

CSI247Mu01 5X10^5
EUR 860

OASA11544-100T - MACROPHAGES:RPE Antibody

OASA11544-100T 100Tests
EUR 479

Mouse anti macrophage antibody ELISA kit

E03A2088-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Mouse anti macrophage antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse anti macrophage antibody ELISA kit

E03A2088-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Mouse anti macrophage antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
The DP plus BMP-2 (DPB) group confirmed a lot of chondrocytes and partial new bone tissues.  PELA electrospun fibrous scaffolds are good sustained-release carriers, which cannot solely induce implant differentiation into cartilage and bone but additionally are fully degraded with out toxicity. Due to this fact, the fabric can be utilized for bone and cartilage regeneration.
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